computer-assisted sequence analysis Search Results


3d reconstruction image  (Oxford Instruments)
99
Oxford Instruments 3d reconstruction image
MEF2C enhancer mutation impairs MEF2C transcription and leads to mitochondrial dysfunction. (A) Demonstration of CRISPR-editing of rs304152-G mutation in HEK293T cells. Underlined sequence and blue sequence determine sgRNA and PAM, respectively. Scheme created with BioRender.com. (B) MEF2C mRNA level decreased in rs304152-G (MT) cells compared to rs304152-T (WT) cells. Statistics were calculated using Student’s t-test: *, P = 0.047. (C) Immunofluorescence staining of MEF2C (green) and outer mitochondria membrane <t>marker,</t> <t>TOMM20</t> (red), in human cortical pyramidal neuron along with deconvolved and <t>3D</t> reconstruction image made by Imaris 9 (Bitplane). Right panel shows line measurement analysis for MEF2C and TOMM20 colocalization signals. White dotted line indicates the colocalization analysis line. (D) Ultrafractionation of cellular compartments of mouse brain tissues. (E) Western blots of cell fractionation confirmed the presence of MEF2C in mitochondria in pure mitochondrial fractions. (F) qPCR results showed decrease of ND4 mRNA levels in MT cells. Statistics were calculated using Student’s t-test: **, P < 0.002. (G) Immunofluorescence staining of MEF2C and ND4 in WT and MT cells. Scale bars (white): 5 μm. Right: densitometry analysis showed decrease of MEF2C and ND4 levels in MT cells. Scatter plot represents positive correlation between MEF2C and ND4 levels. A total of 39 cells/group were counted (13 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( ***, P < 0.001). (H) Immunostaining of MitoTracker (red) and MitoSox (green) in WT and MT cells. The nuclei were counterstained with DAPI (blue). Right: quantification of MitoTracker and MitoSox levels. A total of 36 cells/group were counted (12 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( *, P = 0.037; ***, P < 0.001). (I) Decrease of ATP level in MT cells compared to WT cells. The experiment was repeated three times. Statistics were calculated using Student’s t-test ( **, P = 0.006). Error bars represent means ± SEM
3d Reconstruction Image, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/computer-assisted+sequence+analysis/pmc11806887-135-32-37?v=Oxford+Instruments
Average 99 stars, based on 1 article reviews
3d reconstruction image - by Bioz Stars, 2026-07
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hif-1 alpha antibody (h1alpha67)  (Bio-Techne corporation)
97
Bio-Techne corporation hif-1 alpha antibody (h1alpha67)
MEF2C enhancer mutation impairs MEF2C transcription and leads to mitochondrial dysfunction. (A) Demonstration of CRISPR-editing of rs304152-G mutation in HEK293T cells. Underlined sequence and blue sequence determine sgRNA and PAM, respectively. Scheme created with BioRender.com. (B) MEF2C mRNA level decreased in rs304152-G (MT) cells compared to rs304152-T (WT) cells. Statistics were calculated using Student’s t-test: *, P = 0.047. (C) Immunofluorescence staining of MEF2C (green) and outer mitochondria membrane <t>marker,</t> <t>TOMM20</t> (red), in human cortical pyramidal neuron along with deconvolved and <t>3D</t> reconstruction image made by Imaris 9 (Bitplane). Right panel shows line measurement analysis for MEF2C and TOMM20 colocalization signals. White dotted line indicates the colocalization analysis line. (D) Ultrafractionation of cellular compartments of mouse brain tissues. (E) Western blots of cell fractionation confirmed the presence of MEF2C in mitochondria in pure mitochondrial fractions. (F) qPCR results showed decrease of ND4 mRNA levels in MT cells. Statistics were calculated using Student’s t-test: **, P < 0.002. (G) Immunofluorescence staining of MEF2C and ND4 in WT and MT cells. Scale bars (white): 5 μm. Right: densitometry analysis showed decrease of MEF2C and ND4 levels in MT cells. Scatter plot represents positive correlation between MEF2C and ND4 levels. A total of 39 cells/group were counted (13 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( ***, P < 0.001). (H) Immunostaining of MitoTracker (red) and MitoSox (green) in WT and MT cells. The nuclei were counterstained with DAPI (blue). Right: quantification of MitoTracker and MitoSox levels. A total of 36 cells/group were counted (12 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( *, P = 0.037; ***, P < 0.001). (I) Decrease of ATP level in MT cells compared to WT cells. The experiment was repeated three times. Statistics were calculated using Student’s t-test ( **, P = 0.006). Error bars represent means ± SEM
Hif 1 Alpha Antibody (H1alpha67), supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/computer-assisted+sequence+analysis/bio-techne+corporation___nb100-105?v=Bio-Techne+corporation
Average 97 stars, based on 1 article reviews
hif-1 alpha antibody (h1alpha67) - by Bioz Stars, 2026-07
97/100 stars
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gene2promoter module  (Genomatix gmbh)
90
Genomatix gmbh gene2promoter module
MEF2C enhancer mutation impairs MEF2C transcription and leads to mitochondrial dysfunction. (A) Demonstration of CRISPR-editing of rs304152-G mutation in HEK293T cells. Underlined sequence and blue sequence determine sgRNA and PAM, respectively. Scheme created with BioRender.com. (B) MEF2C mRNA level decreased in rs304152-G (MT) cells compared to rs304152-T (WT) cells. Statistics were calculated using Student’s t-test: *, P = 0.047. (C) Immunofluorescence staining of MEF2C (green) and outer mitochondria membrane <t>marker,</t> <t>TOMM20</t> (red), in human cortical pyramidal neuron along with deconvolved and <t>3D</t> reconstruction image made by Imaris 9 (Bitplane). Right panel shows line measurement analysis for MEF2C and TOMM20 colocalization signals. White dotted line indicates the colocalization analysis line. (D) Ultrafractionation of cellular compartments of mouse brain tissues. (E) Western blots of cell fractionation confirmed the presence of MEF2C in mitochondria in pure mitochondrial fractions. (F) qPCR results showed decrease of ND4 mRNA levels in MT cells. Statistics were calculated using Student’s t-test: **, P < 0.002. (G) Immunofluorescence staining of MEF2C and ND4 in WT and MT cells. Scale bars (white): 5 μm. Right: densitometry analysis showed decrease of MEF2C and ND4 levels in MT cells. Scatter plot represents positive correlation between MEF2C and ND4 levels. A total of 39 cells/group were counted (13 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( ***, P < 0.001). (H) Immunostaining of MitoTracker (red) and MitoSox (green) in WT and MT cells. The nuclei were counterstained with DAPI (blue). Right: quantification of MitoTracker and MitoSox levels. A total of 36 cells/group were counted (12 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( *, P = 0.037; ***, P < 0.001). (I) Decrease of ATP level in MT cells compared to WT cells. The experiment was repeated three times. Statistics were calculated using Student’s t-test ( **, P = 0.006). Error bars represent means ± SEM
Gene2promoter Module, supplied by Genomatix gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/computer-assisted+sequence+analysis/10__1017_slash_s1751731108001584-51-15-18?v=Genomatix+gmbh
Average 90 stars, based on 1 article reviews
gene2promoter module - by Bioz Stars, 2026-07
90/100 stars
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macvector software package  (MacVector inc)
90
MacVector inc macvector software package
MEF2C enhancer mutation impairs MEF2C transcription and leads to mitochondrial dysfunction. (A) Demonstration of CRISPR-editing of rs304152-G mutation in HEK293T cells. Underlined sequence and blue sequence determine sgRNA and PAM, respectively. Scheme created with BioRender.com. (B) MEF2C mRNA level decreased in rs304152-G (MT) cells compared to rs304152-T (WT) cells. Statistics were calculated using Student’s t-test: *, P = 0.047. (C) Immunofluorescence staining of MEF2C (green) and outer mitochondria membrane <t>marker,</t> <t>TOMM20</t> (red), in human cortical pyramidal neuron along with deconvolved and <t>3D</t> reconstruction image made by Imaris 9 (Bitplane). Right panel shows line measurement analysis for MEF2C and TOMM20 colocalization signals. White dotted line indicates the colocalization analysis line. (D) Ultrafractionation of cellular compartments of mouse brain tissues. (E) Western blots of cell fractionation confirmed the presence of MEF2C in mitochondria in pure mitochondrial fractions. (F) qPCR results showed decrease of ND4 mRNA levels in MT cells. Statistics were calculated using Student’s t-test: **, P < 0.002. (G) Immunofluorescence staining of MEF2C and ND4 in WT and MT cells. Scale bars (white): 5 μm. Right: densitometry analysis showed decrease of MEF2C and ND4 levels in MT cells. Scatter plot represents positive correlation between MEF2C and ND4 levels. A total of 39 cells/group were counted (13 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( ***, P < 0.001). (H) Immunostaining of MitoTracker (red) and MitoSox (green) in WT and MT cells. The nuclei were counterstained with DAPI (blue). Right: quantification of MitoTracker and MitoSox levels. A total of 36 cells/group were counted (12 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( *, P = 0.037; ***, P < 0.001). (I) Decrease of ATP level in MT cells compared to WT cells. The experiment was repeated three times. Statistics were calculated using Student’s t-test ( **, P = 0.006). Error bars represent means ± SEM
Macvector Software Package, supplied by MacVector inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/computer-assisted+sequence+analysis/pmc02749447-202-9-9?v=MacVector+inc
Average 90 stars, based on 1 article reviews
macvector software package - by Bioz Stars, 2026-07
90/100 stars
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lasergene bio computing software  (DNASTAR)
99
DNASTAR lasergene bio computing software
MEF2C enhancer mutation impairs MEF2C transcription and leads to mitochondrial dysfunction. (A) Demonstration of CRISPR-editing of rs304152-G mutation in HEK293T cells. Underlined sequence and blue sequence determine sgRNA and PAM, respectively. Scheme created with BioRender.com. (B) MEF2C mRNA level decreased in rs304152-G (MT) cells compared to rs304152-T (WT) cells. Statistics were calculated using Student’s t-test: *, P = 0.047. (C) Immunofluorescence staining of MEF2C (green) and outer mitochondria membrane <t>marker,</t> <t>TOMM20</t> (red), in human cortical pyramidal neuron along with deconvolved and <t>3D</t> reconstruction image made by Imaris 9 (Bitplane). Right panel shows line measurement analysis for MEF2C and TOMM20 colocalization signals. White dotted line indicates the colocalization analysis line. (D) Ultrafractionation of cellular compartments of mouse brain tissues. (E) Western blots of cell fractionation confirmed the presence of MEF2C in mitochondria in pure mitochondrial fractions. (F) qPCR results showed decrease of ND4 mRNA levels in MT cells. Statistics were calculated using Student’s t-test: **, P < 0.002. (G) Immunofluorescence staining of MEF2C and ND4 in WT and MT cells. Scale bars (white): 5 μm. Right: densitometry analysis showed decrease of MEF2C and ND4 levels in MT cells. Scatter plot represents positive correlation between MEF2C and ND4 levels. A total of 39 cells/group were counted (13 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( ***, P < 0.001). (H) Immunostaining of MitoTracker (red) and MitoSox (green) in WT and MT cells. The nuclei were counterstained with DAPI (blue). Right: quantification of MitoTracker and MitoSox levels. A total of 36 cells/group were counted (12 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( *, P = 0.037; ***, P < 0.001). (I) Decrease of ATP level in MT cells compared to WT cells. The experiment was repeated three times. Statistics were calculated using Student’s t-test ( **, P = 0.006). Error bars represent means ± SEM
Lasergene Bio Computing Software, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/computer-assisted+sequence+analysis/pmc00224574-160-9-12?v=DNASTAR
Average 99 stars, based on 1 article reviews
lasergene bio computing software - by Bioz Stars, 2026-07
99/100 stars
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contigexpress software package  (InforMax Inc)
90
InforMax Inc contigexpress software package
MEF2C enhancer mutation impairs MEF2C transcription and leads to mitochondrial dysfunction. (A) Demonstration of CRISPR-editing of rs304152-G mutation in HEK293T cells. Underlined sequence and blue sequence determine sgRNA and PAM, respectively. Scheme created with BioRender.com. (B) MEF2C mRNA level decreased in rs304152-G (MT) cells compared to rs304152-T (WT) cells. Statistics were calculated using Student’s t-test: *, P = 0.047. (C) Immunofluorescence staining of MEF2C (green) and outer mitochondria membrane <t>marker,</t> <t>TOMM20</t> (red), in human cortical pyramidal neuron along with deconvolved and <t>3D</t> reconstruction image made by Imaris 9 (Bitplane). Right panel shows line measurement analysis for MEF2C and TOMM20 colocalization signals. White dotted line indicates the colocalization analysis line. (D) Ultrafractionation of cellular compartments of mouse brain tissues. (E) Western blots of cell fractionation confirmed the presence of MEF2C in mitochondria in pure mitochondrial fractions. (F) qPCR results showed decrease of ND4 mRNA levels in MT cells. Statistics were calculated using Student’s t-test: **, P < 0.002. (G) Immunofluorescence staining of MEF2C and ND4 in WT and MT cells. Scale bars (white): 5 μm. Right: densitometry analysis showed decrease of MEF2C and ND4 levels in MT cells. Scatter plot represents positive correlation between MEF2C and ND4 levels. A total of 39 cells/group were counted (13 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( ***, P < 0.001). (H) Immunostaining of MitoTracker (red) and MitoSox (green) in WT and MT cells. The nuclei were counterstained with DAPI (blue). Right: quantification of MitoTracker and MitoSox levels. A total of 36 cells/group were counted (12 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( *, P = 0.037; ***, P < 0.001). (I) Decrease of ATP level in MT cells compared to WT cells. The experiment was repeated three times. Statistics were calculated using Student’s t-test ( **, P = 0.006). Error bars represent means ± SEM
Contigexpress Software Package, supplied by InforMax Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/computer-assisted+sequence+analysis/pmc00368321-193-8-11?v=InforMax+Inc
Average 90 stars, based on 1 article reviews
contigexpress software package - by Bioz Stars, 2026-07
90/100 stars
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computer-assisted sequence analysis  (Genomatix gmbh)
90
Genomatix gmbh computer-assisted sequence analysis
MEF2C enhancer mutation impairs MEF2C transcription and leads to mitochondrial dysfunction. (A) Demonstration of CRISPR-editing of rs304152-G mutation in HEK293T cells. Underlined sequence and blue sequence determine sgRNA and PAM, respectively. Scheme created with BioRender.com. (B) MEF2C mRNA level decreased in rs304152-G (MT) cells compared to rs304152-T (WT) cells. Statistics were calculated using Student’s t-test: *, P = 0.047. (C) Immunofluorescence staining of MEF2C (green) and outer mitochondria membrane <t>marker,</t> <t>TOMM20</t> (red), in human cortical pyramidal neuron along with deconvolved and <t>3D</t> reconstruction image made by Imaris 9 (Bitplane). Right panel shows line measurement analysis for MEF2C and TOMM20 colocalization signals. White dotted line indicates the colocalization analysis line. (D) Ultrafractionation of cellular compartments of mouse brain tissues. (E) Western blots of cell fractionation confirmed the presence of MEF2C in mitochondria in pure mitochondrial fractions. (F) qPCR results showed decrease of ND4 mRNA levels in MT cells. Statistics were calculated using Student’s t-test: **, P < 0.002. (G) Immunofluorescence staining of MEF2C and ND4 in WT and MT cells. Scale bars (white): 5 μm. Right: densitometry analysis showed decrease of MEF2C and ND4 levels in MT cells. Scatter plot represents positive correlation between MEF2C and ND4 levels. A total of 39 cells/group were counted (13 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( ***, P < 0.001). (H) Immunostaining of MitoTracker (red) and MitoSox (green) in WT and MT cells. The nuclei were counterstained with DAPI (blue). Right: quantification of MitoTracker and MitoSox levels. A total of 36 cells/group were counted (12 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( *, P = 0.037; ***, P < 0.001). (I) Decrease of ATP level in MT cells compared to WT cells. The experiment was repeated three times. Statistics were calculated using Student’s t-test ( **, P = 0.006). Error bars represent means ± SEM
Computer Assisted Sequence Analysis, supplied by Genomatix gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/computer-assisted+sequence+analysis/10__1017_slash_s1751731108001584-104-2-9?v=Genomatix+gmbh
Average 90 stars, based on 1 article reviews
computer-assisted sequence analysis - by Bioz Stars, 2026-07
90/100 stars
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dna sequence analysis program macvector 9  (MacVector inc)
90
MacVector inc dna sequence analysis program macvector 9
MEF2C enhancer mutation impairs MEF2C transcription and leads to mitochondrial dysfunction. (A) Demonstration of CRISPR-editing of rs304152-G mutation in HEK293T cells. Underlined sequence and blue sequence determine sgRNA and PAM, respectively. Scheme created with BioRender.com. (B) MEF2C mRNA level decreased in rs304152-G (MT) cells compared to rs304152-T (WT) cells. Statistics were calculated using Student’s t-test: *, P = 0.047. (C) Immunofluorescence staining of MEF2C (green) and outer mitochondria membrane <t>marker,</t> <t>TOMM20</t> (red), in human cortical pyramidal neuron along with deconvolved and <t>3D</t> reconstruction image made by Imaris 9 (Bitplane). Right panel shows line measurement analysis for MEF2C and TOMM20 colocalization signals. White dotted line indicates the colocalization analysis line. (D) Ultrafractionation of cellular compartments of mouse brain tissues. (E) Western blots of cell fractionation confirmed the presence of MEF2C in mitochondria in pure mitochondrial fractions. (F) qPCR results showed decrease of ND4 mRNA levels in MT cells. Statistics were calculated using Student’s t-test: **, P < 0.002. (G) Immunofluorescence staining of MEF2C and ND4 in WT and MT cells. Scale bars (white): 5 μm. Right: densitometry analysis showed decrease of MEF2C and ND4 levels in MT cells. Scatter plot represents positive correlation between MEF2C and ND4 levels. A total of 39 cells/group were counted (13 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( ***, P < 0.001). (H) Immunostaining of MitoTracker (red) and MitoSox (green) in WT and MT cells. The nuclei were counterstained with DAPI (blue). Right: quantification of MitoTracker and MitoSox levels. A total of 36 cells/group were counted (12 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( *, P = 0.037; ***, P < 0.001). (I) Decrease of ATP level in MT cells compared to WT cells. The experiment was repeated three times. Statistics were calculated using Student’s t-test ( **, P = 0.006). Error bars represent means ± SEM
Dna Sequence Analysis Program Macvector 9, supplied by MacVector inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/computer-assisted+sequence+analysis/pmc02817632-81-10-15?v=MacVector+inc
Average 90 stars, based on 1 article reviews
dna sequence analysis program macvector 9 - by Bioz Stars, 2026-07
90/100 stars
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dna polymerase i  (New England Biolabs)
99
New England Biolabs dna polymerase i
MEF2C enhancer mutation impairs MEF2C transcription and leads to mitochondrial dysfunction. (A) Demonstration of CRISPR-editing of rs304152-G mutation in HEK293T cells. Underlined sequence and blue sequence determine sgRNA and PAM, respectively. Scheme created with BioRender.com. (B) MEF2C mRNA level decreased in rs304152-G (MT) cells compared to rs304152-T (WT) cells. Statistics were calculated using Student’s t-test: *, P = 0.047. (C) Immunofluorescence staining of MEF2C (green) and outer mitochondria membrane <t>marker,</t> <t>TOMM20</t> (red), in human cortical pyramidal neuron along with deconvolved and <t>3D</t> reconstruction image made by Imaris 9 (Bitplane). Right panel shows line measurement analysis for MEF2C and TOMM20 colocalization signals. White dotted line indicates the colocalization analysis line. (D) Ultrafractionation of cellular compartments of mouse brain tissues. (E) Western blots of cell fractionation confirmed the presence of MEF2C in mitochondria in pure mitochondrial fractions. (F) qPCR results showed decrease of ND4 mRNA levels in MT cells. Statistics were calculated using Student’s t-test: **, P < 0.002. (G) Immunofluorescence staining of MEF2C and ND4 in WT and MT cells. Scale bars (white): 5 μm. Right: densitometry analysis showed decrease of MEF2C and ND4 levels in MT cells. Scatter plot represents positive correlation between MEF2C and ND4 levels. A total of 39 cells/group were counted (13 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( ***, P < 0.001). (H) Immunostaining of MitoTracker (red) and MitoSox (green) in WT and MT cells. The nuclei were counterstained with DAPI (blue). Right: quantification of MitoTracker and MitoSox levels. A total of 36 cells/group were counted (12 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( *, P = 0.037; ***, P < 0.001). (I) Decrease of ATP level in MT cells compared to WT cells. The experiment was repeated three times. Statistics were calculated using Student’s t-test ( **, P = 0.006). Error bars represent means ± SEM
Dna Polymerase I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/computer-assisted+sequence+analysis/custom%40m0209%4010610717?v=New+England+Biolabs
Average 99 stars, based on 1 article reviews
dna polymerase i - by Bioz Stars, 2026-07
99/100 stars
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klenow fragment  (New England Biolabs)
99
New England Biolabs klenow fragment
MEF2C enhancer mutation impairs MEF2C transcription and leads to mitochondrial dysfunction. (A) Demonstration of CRISPR-editing of rs304152-G mutation in HEK293T cells. Underlined sequence and blue sequence determine sgRNA and PAM, respectively. Scheme created with BioRender.com. (B) MEF2C mRNA level decreased in rs304152-G (MT) cells compared to rs304152-T (WT) cells. Statistics were calculated using Student’s t-test: *, P = 0.047. (C) Immunofluorescence staining of MEF2C (green) and outer mitochondria membrane <t>marker,</t> <t>TOMM20</t> (red), in human cortical pyramidal neuron along with deconvolved and <t>3D</t> reconstruction image made by Imaris 9 (Bitplane). Right panel shows line measurement analysis for MEF2C and TOMM20 colocalization signals. White dotted line indicates the colocalization analysis line. (D) Ultrafractionation of cellular compartments of mouse brain tissues. (E) Western blots of cell fractionation confirmed the presence of MEF2C in mitochondria in pure mitochondrial fractions. (F) qPCR results showed decrease of ND4 mRNA levels in MT cells. Statistics were calculated using Student’s t-test: **, P < 0.002. (G) Immunofluorescence staining of MEF2C and ND4 in WT and MT cells. Scale bars (white): 5 μm. Right: densitometry analysis showed decrease of MEF2C and ND4 levels in MT cells. Scatter plot represents positive correlation between MEF2C and ND4 levels. A total of 39 cells/group were counted (13 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( ***, P < 0.001). (H) Immunostaining of MitoTracker (red) and MitoSox (green) in WT and MT cells. The nuclei were counterstained with DAPI (blue). Right: quantification of MitoTracker and MitoSox levels. A total of 36 cells/group were counted (12 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( *, P = 0.037; ***, P < 0.001). (I) Decrease of ATP level in MT cells compared to WT cells. The experiment was repeated three times. Statistics were calculated using Student’s t-test ( **, P = 0.006). Error bars represent means ± SEM
Klenow Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/computer-assisted+sequence+analysis/custom%40m0212%4010610717?v=New+England+Biolabs
Average 99 stars, based on 1 article reviews
klenow fragment - by Bioz Stars, 2026-07
99/100 stars
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alignx v.6.0  (InforMax Inc)
90
InforMax Inc alignx v.6.0
MEF2C enhancer mutation impairs MEF2C transcription and leads to mitochondrial dysfunction. (A) Demonstration of CRISPR-editing of rs304152-G mutation in HEK293T cells. Underlined sequence and blue sequence determine sgRNA and PAM, respectively. Scheme created with BioRender.com. (B) MEF2C mRNA level decreased in rs304152-G (MT) cells compared to rs304152-T (WT) cells. Statistics were calculated using Student’s t-test: *, P = 0.047. (C) Immunofluorescence staining of MEF2C (green) and outer mitochondria membrane <t>marker,</t> <t>TOMM20</t> (red), in human cortical pyramidal neuron along with deconvolved and <t>3D</t> reconstruction image made by Imaris 9 (Bitplane). Right panel shows line measurement analysis for MEF2C and TOMM20 colocalization signals. White dotted line indicates the colocalization analysis line. (D) Ultrafractionation of cellular compartments of mouse brain tissues. (E) Western blots of cell fractionation confirmed the presence of MEF2C in mitochondria in pure mitochondrial fractions. (F) qPCR results showed decrease of ND4 mRNA levels in MT cells. Statistics were calculated using Student’s t-test: **, P < 0.002. (G) Immunofluorescence staining of MEF2C and ND4 in WT and MT cells. Scale bars (white): 5 μm. Right: densitometry analysis showed decrease of MEF2C and ND4 levels in MT cells. Scatter plot represents positive correlation between MEF2C and ND4 levels. A total of 39 cells/group were counted (13 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( ***, P < 0.001). (H) Immunostaining of MitoTracker (red) and MitoSox (green) in WT and MT cells. The nuclei were counterstained with DAPI (blue). Right: quantification of MitoTracker and MitoSox levels. A total of 36 cells/group were counted (12 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( *, P = 0.037; ***, P < 0.001). (I) Decrease of ATP level in MT cells compared to WT cells. The experiment was repeated three times. Statistics were calculated using Student’s t-test ( **, P = 0.006). Error bars represent means ± SEM
Alignx V.6.0, supplied by InforMax Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/computer-assisted+sequence+analysis/pm16254445-51-0-5?v=InforMax+Inc
Average 90 stars, based on 1 article reviews
alignx v.6.0 - by Bioz Stars, 2026-07
90/100 stars
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MEF2C enhancer mutation impairs MEF2C transcription and leads to mitochondrial dysfunction. (A) Demonstration of CRISPR-editing of rs304152-G mutation in HEK293T cells. Underlined sequence and blue sequence determine sgRNA and PAM, respectively. Scheme created with BioRender.com. (B) MEF2C mRNA level decreased in rs304152-G (MT) cells compared to rs304152-T (WT) cells. Statistics were calculated using Student’s t-test: *, P = 0.047. (C) Immunofluorescence staining of MEF2C (green) and outer mitochondria membrane marker, TOMM20 (red), in human cortical pyramidal neuron along with deconvolved and 3D reconstruction image made by Imaris 9 (Bitplane). Right panel shows line measurement analysis for MEF2C and TOMM20 colocalization signals. White dotted line indicates the colocalization analysis line. (D) Ultrafractionation of cellular compartments of mouse brain tissues. (E) Western blots of cell fractionation confirmed the presence of MEF2C in mitochondria in pure mitochondrial fractions. (F) qPCR results showed decrease of ND4 mRNA levels in MT cells. Statistics were calculated using Student’s t-test: **, P < 0.002. (G) Immunofluorescence staining of MEF2C and ND4 in WT and MT cells. Scale bars (white): 5 μm. Right: densitometry analysis showed decrease of MEF2C and ND4 levels in MT cells. Scatter plot represents positive correlation between MEF2C and ND4 levels. A total of 39 cells/group were counted (13 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( ***, P < 0.001). (H) Immunostaining of MitoTracker (red) and MitoSox (green) in WT and MT cells. The nuclei were counterstained with DAPI (blue). Right: quantification of MitoTracker and MitoSox levels. A total of 36 cells/group were counted (12 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( *, P = 0.037; ***, P < 0.001). (I) Decrease of ATP level in MT cells compared to WT cells. The experiment was repeated three times. Statistics were calculated using Student’s t-test ( **, P = 0.006). Error bars represent means ± SEM

Journal: Molecular Neurodegeneration

Article Title: Loss of MEF2C function by enhancer mutation leads to neuronal mitochondria dysfunction and motor deficits in mice

doi: 10.1186/s13024-024-00792-y

Figure Lengend Snippet: MEF2C enhancer mutation impairs MEF2C transcription and leads to mitochondrial dysfunction. (A) Demonstration of CRISPR-editing of rs304152-G mutation in HEK293T cells. Underlined sequence and blue sequence determine sgRNA and PAM, respectively. Scheme created with BioRender.com. (B) MEF2C mRNA level decreased in rs304152-G (MT) cells compared to rs304152-T (WT) cells. Statistics were calculated using Student’s t-test: *, P = 0.047. (C) Immunofluorescence staining of MEF2C (green) and outer mitochondria membrane marker, TOMM20 (red), in human cortical pyramidal neuron along with deconvolved and 3D reconstruction image made by Imaris 9 (Bitplane). Right panel shows line measurement analysis for MEF2C and TOMM20 colocalization signals. White dotted line indicates the colocalization analysis line. (D) Ultrafractionation of cellular compartments of mouse brain tissues. (E) Western blots of cell fractionation confirmed the presence of MEF2C in mitochondria in pure mitochondrial fractions. (F) qPCR results showed decrease of ND4 mRNA levels in MT cells. Statistics were calculated using Student’s t-test: **, P < 0.002. (G) Immunofluorescence staining of MEF2C and ND4 in WT and MT cells. Scale bars (white): 5 μm. Right: densitometry analysis showed decrease of MEF2C and ND4 levels in MT cells. Scatter plot represents positive correlation between MEF2C and ND4 levels. A total of 39 cells/group were counted (13 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( ***, P < 0.001). (H) Immunostaining of MitoTracker (red) and MitoSox (green) in WT and MT cells. The nuclei were counterstained with DAPI (blue). Right: quantification of MitoTracker and MitoSox levels. A total of 36 cells/group were counted (12 cells/well) from n = 3 wells/group (WT and MT). Statistics were calculated using LMM ( *, P = 0.037; ***, P < 0.001). (I) Decrease of ATP level in MT cells compared to WT cells. The experiment was repeated three times. Statistics were calculated using Student’s t-test ( **, P = 0.006). Error bars represent means ± SEM

Article Snippet: Statistics were calculated using Student’s t-test: *, P = 0.047. (C) Immunofluorescence staining of MEF2C (green) and outer mitochondria membrane marker, TOMM20 (red), in human cortical pyramidal neuron along with deconvolved and 3D reconstruction image made by Imaris 9 (Bitplane).

Techniques: Mutagenesis, CRISPR, Sequencing, Immunofluorescence, Staining, Membrane, Marker, Western Blot, Cell Fractionation, Immunostaining